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Realised by ALMS™
developer of the AIDS-HIV Reference project
Abstract No.: MoP-037
Session: Food and Nutrition
Presentation date: Mon, Aug 28, 2006
Presentation time: 09:50 – 11:20

GC/MS Analysis of Microbial Metabolites from Flavanols in an In Vitro Human Colon Model

Ismo Mattila1, Tuulikki Seppanen-Laakso1, Anna-Marja Aura1

1 VTT, Espoo, Finland

Correspondence address: Ismo Mattila, VTT, Tietotie 2, Espoo, 02230 Finland.

Keywords: Acids, Organic; Mass Spectrometry, Gas Chromatography; Metabolism, Metabolites; Phenols.

Novel aspect: Method optimization for the analysis of microbial metabolites.

 

Dietary phenolic compounds are plant derived secondary metabolites, which can be divided into flavonoids, phenolic acids, stilbenes, tannins and lignans. Catechins can be found as monomers or in condensed forms as proanthocyanidins (PA). Pure (+)-catechin, (-)-epicatechin and isolated PA fractions with different chain lengths were fermented with pooled human fecal microbiota in strictly anaerobic conditions. GC/MS analytical method was developed for the analysis of microbial metabolites.

We will describe a GC/MS analytical method and the preceding sample preparation steps for the analysis of 11 microbial metabolites of (+)–catechin and (-)-epicatechin. The compounds include e.g. hydroxyphenyl propionic and acetic acid derivatives. The sample preparation steps include sample extraction (wet or dry) with ethyl acetate and silylation with MSTFA. The fermentation and calibration samples are run in SIM mode and quantified using an internal standard. Different extraction methods and chromatographical methods are compared.

Financial support of the project STREP-FLAVO (Food-CT-2004-513960) is gratefully acknowledged.