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Realised by ALMS™
developer of the AIDS-HIV Reference project
Abstract No.: MoP-160
Session: Metabolomics, Metabonomics
Presentation date: Mon, Aug 28, 2006
Presentation time: 14:30 – 16:00

Semi-quantitative Electrospray Ionization Response for Drugs and Metabolites using Nanoelectrospray for High-flow Liquid Chromatography

Mark Baumert1, Reinaldo Almeida1, Mark Allen1, Gary A. Schultz2, Lawrence Klecha2, Victor Italiano2, Michael Lees2, Peter Weisz2, Jenny Lesinski2, Simon Prosser2

1 Advion, Norwich, United Kingdom
2 Advion BioSystems, Ithaca, United States

Correspondence address: Mark Baumert, Advion, Rowan House, 26-28 Queens Road, Norwich, NR3 9DB United Kingdom.

Keywords: Electrospray Ionization (ESI); Mass Spectrometry; Metabolism, Metabolites; Nanoscale Science/Technology.

Novel aspect: Simultaneous LC/MS and fraction collection.

 

LC-MS continues to be the gold standard for acquiring qualitative and quantitative information for complex mixtures. Applications such as metabolomics, biomarkers and proteomics involve the analysis of known and unknown species with a desire to quantify and characterize each component. Sample complexity limits the amount of time for MS/MS and MSn during an LC run. This work will describe the development of a system that enables coupling of LC columns with flow rates up to 1.0 mL/min to the ESI Chip with use of a post-column splitter. The system enables collection of LC-MS and LC fractions simultaneously. Software links LC-MS retention times to specific LC fractions collected in 96 or 384-well sample plates. Infusion of LC fractions enables optimization of MS/MS parameters such as collision energy for complex, multi-component fractions demonstrating greater than 10 times improvement in S/N compared to online LC-MS/MS. Ibuprofen metabolites in human urine were investigated by both online LC/MS and chip-based infusion nanoESI of LC fractions. This work will highlight the improved data obtained by extended analysis of LC fractions for obtaining MS/MS data on low level metabolites.