17th International Mass Spectrometry Conference :: Prague, 2006
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|Session:||Clinical Chemistry Applications|
|Presentation date:||Tue, Aug 29, 2006|
|Presentation time:||14:30 – 16:00|
Pekka Keski-Rahkonen1, Olavi Parssinen2, Esa Leppanen3, Timo Mauriala1, Marko Lehtonen1, Seppo Auriola11 Department of Pharmaceutical Chemistry, University of Kuopio, Kuopio, Finland
Correspondence address: Pekka Keski-Rahkonen, University of Kuopio, Department of Pharmaceutical Chemistry, Harjulantie 1, Kuopio, 70210 Finland.
Keywords: Electrospray Ionization (ESI); Mass Spectrometry, Ion Trap Quadrupole; Pharmaceutical; Quantitative Analysis.
Novel aspect: Determination of tamsulosin in human aqueous humor.
Tamsulosin is the most frequently prescribed medication for the treatment of lower urinary tract symptoms of benign prostatic hyperplasia (BPH). However, recent findings suggest that there is a strong association between systemic use of tamsulosin and iris hypotony or intraoperative floppy iris syndrome (IFIS). In order to study these eye-related adverse effects of tamsulosin, a simple, sensitive and specific LC-MS/MS method was developed for its determination in human aqueous humor and serum. Aqueous humor samples were analyzed by direct injection, after addition of the internal standard, labetalol. Liquid-liquid extraction with ethyl acetate was used for serum sample preparation. The chromatographic separation was performed on a reversed phase column by gradient elution with acetonitrile - 0.1 % formic acid at a flow-rate of 0.2 ml/min. Detection and quantification of the analytes were carried out with a linear ion trap mass spectrometer, using positive electrospray ionization (ESI) and multiple reaction monitoring (MRM). The limit of quantification was 0.1 ng/ml for both aqueous humor and serum samples and linearity was obtained over the concentration ranges of 0.1 - 4.7 ng/ml and 0.1 - 20 ng/ml for aqueous humor and serum samples, respectively. Acceptable accuracy and precision were obtained for concentrations within the standard curve ranges. The method has been used for the determination of tamsulosin in aqueous humor and serum samples from patients that were on tamsulosin medication and underwent cataract surgery.