17th International Mass Spectrometry Conference :: Prague, 2006
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|Session:||LATE-BREAKING/Food and Nutrition|
|Presentation date:||Mon, Aug 28, 2006|
|Presentation time:||09:50 – 11:20|
Maurizio Fiori1, Carolina Ferranti1, Fernanda Delli Quadri1, Giovanni Ferretti1, Rosa Draisci, Luca Palleschi1 Istituto Superiore di Sanita, Rome, Italy
Correspondence address: Maurizio Fiori, Istituto Superiore di Sanita, Food Safety and Veterinary Pubblic Health, Viale Regina Elena 299, Rome, 00161 Italy.
Keywords: MS/MS, Liquid Chromatography; Quantitative Analysis; Separations; Steroids.
Novel aspect: A novel confirmatory method for the determination of natural hormones according to Commission Decision 2002/657/EC and the Italian legislation on natural hormones.
A recently in-house validated LC-MS/MS method for the separation, identification and quantification of residues of 17α-estradiol (17α-E2) and 17β-estradiol (17β-E2) in bovine serum is reported. To our knowledge, no citations are in literature concerning the chromatographic separation of those analytes. The method, validated according to Commission Decision 2002/657/EC, is routinely used in the Italian National Reference Laboratory for residues for the confirmatory analysis. The obtained decision limits (CCα) were of 0.06 ng/mL and 0.03 ng/mL, respectively for 17α-E2 and 17β-E2. Those values are in conformity with the Italian legislation on natural hormones. Detection capabilities (CC&beta) were obtained of 0.08 ng/mL and 0.04 ng/mL, respectively for 17α-E2 and 17β-E2. The confirmatory method was also validated through the determination of precision, accuracy, specificity, stability and calibration curve. Recovery data ranged from 86.3% to 93.2%. The method was specific and robust for the required purposes.