17th International Mass Spectrometry Conference :: Prague, 2006
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|Presentation date:||Mon, Aug 28, 2006|
|Presentation time:||09:50 – 11:20|
Rumi Kim1, Jong Suk Lee1, Dockyu Kim1, Jihyun F. Kim1, Seung-Hwan Park1, Choong-Hwan Lee11 Korea Reaseach Institute of Bioscience and Biotechnology, Daejeon, Korea
Correspondence address: Rumi Kim, Korea Reaseach Institute of Bioscience and Biotechnology, 52 Yeoeun-dong, Yusung-ku, Daejeon, 305-333 Korea.
Web site: http://www.microbank.re.kr
Keywords: Ion, Multiply Charged; Ion, Product; Metabolic Profiling; MS/MS.
Novel aspect: Development and application of LC-MS instrumentation to the analysis of microbolic footprinting for the metabolite profiling.
Paenibacillus polymyxa strains, which are generally recognized as plant growth promoting rhizobacteria (PGPR), produce a wide variety of secondary metaboltes, including plant growth-regulating substances, such as auxin and cytokinin, including antagonists such as polymyxin, fusaricidin, and chitinase. P. polymyxa strain E681, isolated from the rhizosphere of winter crops, produces antagonistic factors such as polymyxin M (mattacin), fusaricidin A•B, and LI-F complexs. And metabolomics consists of strategies to quantitatively identify cellular metabolites and to understand how trafficking of these biochemical messengers through the metabolic network influences influences phenotype. The application of metabolomics to P. polymyxa E681 has been pursued because this organism is used for the production of chemicals, is well known for full genome sequencing. In this research, we show that metabolic footpriting of P. polymyxa E681 was obtained from each culture in the 7 different production media used. Chemical profiles of the extracts were obtained by LC/MS. The 7 different medium could be discriminated either by ions corresponding to known or unknown metabolites. The several metabolites were isolated from the culture medium and further purified by Amberlite XAD-16, and reverse-phase, and size exclusion chromatography. The purified metabolites could be separated into some active compounds and non-target compounds, and characterized by amino acid analysis and combined reverse-phase chromatography and mass spectrometry (LC/MS and MS/MS). The analysis of LC/MS was demonstrated that ions corresponding to the protonated molecular ions (M+H+) from most of the known secondary metabolites and other unknown metabolites produced this bacteria by could observed in the ESI spectra. The different nutrients tested, qualitatively and quantitatively modified the production of metabolites by P. polymyxa E681.