17th International Mass Spectrometry Conference :: Prague, 2006
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|Presentation date:||Thu, Aug 31, 2006|
|Presentation time:||14:30 – 16:00|
Joseph Anacleto1, Sandra Chu1, Byron Kieser1, Yves LeBlanc1, Gary Impey11 Applied Biosystems/MDS Sciex, Concord, Canada
Correspondence address: Joseph Anacleto, Applied Biosystems/MDS Sciex, 71 Four Valley Drive, Concord, L4K 4V8 Canada.
Keywords: Chromatography, Liquid -, Capillary; High Throughput; MS/MS, Liquid Chromatography; Pesticides.
Novel aspect: Using low flow chromatography for large scale MRM screening of compounds in a single analysis.
Currently more than 800 pesticides are used worldwide. For most of these pesticide compounds, regulatory guidelines proscribe maximum residue levels (MRL) in drinking water and food to help protect the community against contamination and the potential negative health effects. These pesticide levels have to be monitored and for this type of target analysis, screening efficiency must be maximized without sacrificing data quality. As part of a hypothetical LC/MS/MS screening technique, a multi-residue screening method was developed for high throughput screening. Samples in various matrices including water, soil, and food products were analyzed for the presence of any of several hundred pesticides used worldwide. This high throughput screening technique used Scheduled MRM transitions monitored throughout a chromatographic run. The number of points across a chromatographic peak is a key determinant of data quality and scheduled MRM transitions enabled improved data quality by increasing the number of points across the peak, relative to conventional approaches, under fast LC conditions. A Tempo™ ht LC system was used for chromatographic elution at a flow rate of 30 ÁL/min through a 0.3 x 50 mm, 3 Ám C18 column. All experiments were performed on a 4000 Q TRAP® LC/MS/MS system. The combination of scheduled MRM analysis with fast chromatography, enabled the overall throughput of this type of screening to be significantly improved.